The influence of different glycosylation patterns on factor VII biological activity

Sutkevičiūtė, Ieva; Mistinienė, Edita; Sereikaitė, Jolanta; Bumelis, Vladas Algirdas

doi:10.1016/j.biochi.2009.05.015

Use this URL to cite this publication: https://hdl.handle.net/20.500.14911/124161

The influence of different glycosylation patterns on factor VII biological activity

Details

Publication Type (CRIS)

Straipsnis Web of Science duomenų bazėje / Article in Web of Science database (S1a)

Publication Type (eLABa)

Straipsnis Web of Science DB / Web of Science DB article (S1)

Author(s)

Author	Affiliation
Sutkevičiūtė, Ieva	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Mistinienė, Edita	UAB SICOR Biotech, Vilnius
Sereikaitė, Jolanta	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Bumelis, Vladas Algirdas	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)

Title

The influence of different glycosylation patterns on factor VII biological activity

[en]

I. Sutkevičiūtė, E. Mistinienė, J. Sereikaitė, V.A. Bumelis

Is part of

Biochimie

Published In

Year	Volume	Issue	Start Page	End Page
2009	91	9	1123	1130

Publisher

Elsevier

Publisher (trusted)

Elsevier

Referenced in Database(s)(DBs)

ScienceDirect

Elsevier Biobase

Science Citation Index Expanded (Web of Science)

Extent

p. 1123-1130

URI

URI	Access Rights
https://hdl.handle.net/20.500.14911/124161
Straipsnis	Viso teksto dokumentas (atviroji prieiga) / Full Text Document (Open Access)

DOI

10.1016/j.biochi.2009.05.015

Cited references

Science / Art Area

Gamtos mokslai / Natural Sciences (N)

Field of Science / Art

Biochemija / Biochemistry (N004)

Keywords (en)

Abstract (en)

In this study the bioactivity of three differently glycosylated blood coagulation factor VII (FVII) variants (human plasma FVII, recombinant human FVII produced in CHO and BHK cell cultures) were analyzed and compared. Surface plasmon resonance studies of FVII interaction with soluble and full length TF together with FVII autoactivation assays revealed that BHK-derived FVII has the highest bioactivity, while human plasma and CHO-derived FVII showed very similar bioactivity. The affinity of FVII variants to TF correlates with FVII autoactivation rates - the higher the affinity, the faster the autoactivation rate.