Nanosecond electrochemotherapy using bleomycin or doxorubicin: Influence of pulse amplitude, duration and burst frequency

Radzevičiūtė-Valčiukė, Eivina; Malyško, Veronika; Kulbacka, Julita; Rembiałkowska, Nina; Novickij, Jurij; Girkontaitė, Irutė; Novickij, Vitalij

doi:10.1016/j.bioelechem.2022.108251

Use this URL to cite this publication: https://hdl.handle.net/20.500.14911/113404

Nanosecond electrochemotherapy using bleomycin or doxorubicin: Influence of pulse amplitude, duration and burst frequency

Details

Publication Type (CRIS)

Straipsnis Web of Science ir Scopus duomenų bazėje / Article in Web of Science and Scopus database (S1)

Publication Type (eLABa)

Straipsnis Web of Science DB / Web of Science DB article (S1)

VILNIUS TECH Research Priorities and Topics

Inovatyvios elektroninės sistemos / Innovative Electronic Systems (MC0505)

Lithuanian Intelligent Specialization

Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies (L105)

Author(s)

Author	Affiliation
Radzevičiūtė-Valčiukė, Eivina	Valstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras
Malyško, Veronika	Elektros inžinerijos katedra / Department of Electrical Engineering (0558)
Kulbacka, Julita	Wroclaw Medical University
Rembiałkowska, Nina	Wroclaw Medical University
Novickij, Jurij	Telekomunikacijų institutas / Institute of Telecommunication (0561)
Girkontaitė, Irutė	Valstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras
Novickij, Vitalij	Stiprių magnetinių laukų institutas / Institute of High Magnetic Fields (0555)

Title

Nanosecond electrochemotherapy using bleomycin or doxorubicin: Influence of pulse amplitude, duration and burst frequency

[en]

Eivina Radzevičiūtė, Veronika Malyško-Ptašinskė, Julita Kulbacka, Nina Rembiałkowska, Jurij Novickij, Irutė Girkontaitė, Vitalij Novickij

Is part of

Bioelectrochemistry

Published In

Year	Volume	Issue	Start Page	End Page
2022	148	108251	1	12

Publisher

Lausanne : Elsevier

Publisher (trusted)

Elsevier

Referenced in Database(s)(DBs)

Science Citation Index Expanded (Web of Science)

SCOPUS

Extent

p. 1-12

URI

URI
https://hdl.handle.net/20.500.14911/113404
https://talpykla.elaba.lt/elaba-fedora/objects/elaba:139237487/datastreams/MAIN/content

DOI

10.1016/j.bioelechem.2022.108251

Cited references

Science / Art Area

Technologijos mokslai / Technological sciences (T)

Field of Science / Art

Elektros ir elektronikos inžinerija / Electrical and electronic engineering (T001)

Biologija / Biology (N010)

Keywords (en)

Abstract (en)

Electroporation is a pulsed electric field (PEF) induced phenomenon, which effectiveness varies dependent on pulse parameters. This work focuses on nano-electrochemotherapy with bleomycin and doxorubicin to derive protocols as effective as European Standard Operating Procedures on Electrochemotherapy (ESOPE), which employ conventional microsecond range pulses. As a model, murine Lewis lung carcinoma (LLC1) cell line was used. The effects of pulse duration (100–500 ns), PEF amplitude (6–10 kV/cm) and pulse repetition frequency (10 kHz, 100 kHz, 1 MHz) were studied. A total of 75 ns protocol variations have been used. For detection of cell permeabilization, Yo-Pro-1 and flow cytometry were employed. Cell viability was evaluated 24-, 48-, or 72-hours post-electroporation. Nanosecond parametric protocols resulting in comparable treatment efficiency as ESOPE (1.3 kV/cm × 100 μs × 8) have been proposed. It was shown that high-frequency nanosecond electrochemotherapy with bleomycin or doxorubicin could be an alternative for established ESOPE protocols.