Determination of the dissociation constant and stoichiometry of a complex of the protein interferon a-2b with Cibacron Blue F3G-A

Bumelienė, Žana; Sereikaitė, Jolanta; Bumelis, Vladas Algirdas; Smirnovas, Vytautas; Gedminienė, Genovaitė; Braziūnaitė, Lina; Bajorūnaitė, Laima

Use this URL to cite this publication: https://hdl.handle.net/20.500.14911/134063

Determination of the dissociation constant and stoichiometry of a complex of the protein interferon a-2b with Cibacron Blue F3G-A

Details

Publication Type (CRIS)

Straipsnis Web of Science duomenų bazėje / Article in Web of Science database (S1a)

Publication Type (eLABa)

Straipsnis Web of Science DB / Web of Science DB article (S1)

Author(s)

Author	Affiliation
Bumelienė, Žana	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Sereikaitė, Jolanta	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Bumelis, Vladas Algirdas	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Smirnovas, Vytautas	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Gedminienė, Genovaitė	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Braziūnaitė, Lina	Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences (10)
Bajorūnaitė, Laima	Informacinių technologijų katedra / Department of Information Technologies (1047)

Title

Determination of the dissociation constant and stoichiometry of a complex of the protein interferon a-2b with Cibacron Blue F3G-A

[en]

Zh. Bumeliene, I. Sereikaite, V.-A. Bumelis, V. Smirnovas, G. Gedminiene, L. Braziunaite, E. Bajorunaite

Is part of

Journal of Analytical Chemistry : translated from Zhurnal Analiticheskoi Khimii

Published In

Year	Volume	Issue	Start Page	End Page
2003	58	11	1038	1041

Publisher

New York : Kluwer Academic/Plenum Publishers

Publisher (trusted)

Kluwer Academic/Plenum Publishers

Extent

p. 1038-1041

URI

URI
https://hdl.handle.net/20.500.14911/134063

Science / Art Area

Technologijos mokslai / Technological sciences (T)

Field of Science / Art

Chemijos inžinerija / Chemical engineering (T005)

Abstract (en)

The nonlinear least-squares technique was used for determining the complexation parameters of the recombinant human cytokine interferon a-2b with a pseudobiospecific ligand, Cibacron Blue F3G-A. The dis- sociation constant of the complex and the number of ligand–protein binding sites were found.