Neisseria denitrificans bakterijų mechaninių ardymo metodų palyginimas
| Author | Affiliation |
|---|---|
Stupak, Robert | Kauno technologijos universitetas |
| Year | Start Page | End Page |
|---|---|---|
2011 | 1 | 7 |
| URI | Access Rights |
|---|---|
| https://hdl.handle.net/20.500.14911/133381 | |
| Straipsnis | Viso teksto dokumentas (atviroji prieiga) / Full Text Document (Open Access) |
Darbe nagrinėti rutulinių bakterijų Neisseria denitrificans ardymo mechaniniais metodais dėsningumai. Bakterijos ardytos rutuliniu malūnu, didelio slėgio homogenizatoriumi, mikrosuskystintoju ir ultragarsu, tuo tarpu gauti rezultatai palyginti su kontroliniu fermentiniu ardymo metodu. Remiantis viduląstelinių produktų, mikroskopavimo ir dalelių dydžio matavimo rezultatais nustatyta, jog mikrosuskystintojas efektyviausiai ardo bakterijas bei turi didžiausia našumą ardant gamybiniu mastu. Tuo tarpu ardymo ultragarsu metodas siūlomas ardant mažus biomasės kiekius. Ardymo procesams pritaikyti matematiniai modeliai bei kiekvienam atitinkamai gautos ardymo greičio konstantos 8.13, 19.61, 2.21, 0.45 min-1.
A comparative evaluation of five different cell-disruption methods for the release of intracellular products from Neisseria denitrificans was investigated. The cell disruption methods evaluated in this study were high pressure homogenization (HPH), microfluidiser (MF), batch mode bead milling (BM), ultrasonication (US) and enzymatic lysis. Ultrasonication was found to be the most effective method for the disruption of small cell quantities in terms of energy utilization, time and degree of cell disruption. Microfluidiser (MF), the kind of high pressure homogenizators, was found to be the most effective for the higher cell quantities (up to 5kg per day). The experimental data of the disruption processes was fitted using the first-order rate equation. The latter resulted in determined disruption rate constants for each method: 8.13, 19.61, 2.21, 0.45 min-1, for HPH, MF, US and BM alternatively.