Kinetic study of peroxidase-catalyzed oxidation of 1-hydroxypyrene. Development of a nanomolar hydrogen peroxide detection system
| Author | Affiliation | |
|---|---|---|
Biochemijos institutas | ||
Biochemijos institutas |
| Year | Volume | Issue | Start Page | End Page |
|---|---|---|---|---|
2008 | 3 | 3 | 224 | 232 |
| URI | Access Rights |
|---|---|
| https://hdl.handle.net/20.500.14911/150156 | |
| Straipsnis | Viso teksto dokumentas (atviroji prieiga) / Full Text Document (Open Access) |
Kinetics of 1-hydroxypyrene (1-HP) oxidation catalyzed with recombinant Coprinus cinereus (rCiP) and horseradish (HRP) peroxidases was investigated with a special emphasis for developing a nanomolar hydrogen peroxide (H2O2) detection system. At pH 8.0 the bimolecular constants of 1-HP oxidation with the ferryl compounds of rCiP and HRP were equal to (1.0 +/- 0.3) x 108 M-1 s(-1) and (0.6 +/- 0.2) x 108 M-1 s(-1),respectively. High bimolecular constants and fluorescence quantum yield of 1-HP (0.66) permitted detection as low as 21 nM of H2O2. To optimize the detection system 1-HP oxidation was modeled at steady-state conditions in the range pH 5.0 to pH 8.0. The 1-HP based detection system was compared with the Amplex Red system. The peroxidase-catalyzed 1-HP oxidation system was used for determination of ozone in the air.