Divinyl sulfone as a crosslinking reagent for oligomeric proteins

Sereikaitė, Jolanta; Bassus, Donata; Bobnis, Renata; Dienys, Gervydas; Bumelienė, Žana; Bumelis, Vladas Algirdas

Use this URL to cite this publication: https://hdl.handle.net/20.500.14911/132926

Divinyl sulfone as a crosslinking reagent for oligomeric proteins

Details

Publication Type (CRIS)

Straipsnis Web of Science ir Scopus duomenų bazėje / Article in Web of Science and Scopus database (S1)

Publication Type (eLABa)

Straipsnis Web of Science DB / Web of Science DB article (S1)

Author(s)

Author	Affiliation
Sereikaitė, Jolanta	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Bassus, Donata	Fundamentinių mokslų fakultetas / Faculty of Fundamental Sciences (10)
Bobnis, Renata	Vilniaus universitetas
Dienys, Gervydas	Vilniaus universitetas
Bumelienė, Žana	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)
Bumelis, Vladas Algirdas	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)

Title

Divinyl sulfone as a crosslinking reagent for oligomeric proteins

[en]

Jolanta Sereikaitė, Donata Bassus, Renata Bobnis, Gervydas Dienys, Žana Bumelienė, Vladas Algirdas Bumelis

Is part of

Russian Journal of Bioorganic Chemistry : translated from ''Bioorganicheskaya Khimiya''

Published In

Year	Volume	Issue	Start Page	End Page
2003	29	3	227	230

Publisher

London : Kluwer Academic/Plenum Publishers

Publisher (trusted)

Kluwer Academic/Plenum Publishers

Referenced in Database(s)(DBs)

SCOPUS

SpringerLink

Science Citation Index Expanded (Web of Science)

Extent

p. 227-230

URI

URI	Access Rights
https://hdl.handle.net/20.500.14911/132926
Straipsnis	Viso teksto dokumentas (atviroji prieiga) / Full Text Document (Open Access)

Cited references

Science / Art Area

Gamtos mokslai / Natural Sciences (N)

Field of Science / Art

Chemija / Chemistry (N003)

Keywords (en)

Abstract (en)

The kinetics of the nucleophilic addition reactions of divinyl sulfone to amino groups of glycine and model proteins was studied in aqueous solution at 30°C. The rate constants for glycine, bovine serum albu-min, and a1-casein were (4.84±0.58)x10¹, (2.97± 0.31)x10², and (2.38 ± 0.49)x10² M¹s¹, respectively. Divinyl sulfone was proposed as a crosslinking reagent for the qualitative detection of protein association in solution. The crosslinking capacity of divinyl sulfone was compared to that of 1,3,5-triacryloylhexahydro-s-tri- azine.