Comparative evaluation of cellular uptake of free and liposomal doxorubicin following short term exposure

Zaleskis, Gintaras; Garberytė, Sima; Pavliukevičienė, Božena; Krasko, Jan Aleksander; Skapas, Martynas; Talaikis, Martynas; Darinskas, Adas; Žibutytė, Lavija; Pašukonienė, Vita

doi:10.21873/anticanres.15011

Use this URL to cite this publication: https://hdl.handle.net/20.500.14911/152164

Comparative evaluation of cellular uptake of free and liposomal doxorubicin following short term exposure

Details

Publication Type (CRIS)

Straipsnis Web of Science ir Scopus duomenų bazėje / Article in Web of Science and Scopus database (S1)

Publication Type (eLABa)

Straipsnis Web of Science DB / Web of Science DB article (S1)

VILNIUS TECH Research Priorities and Topics

Ląstelių ir jų biologiškai aktyvių komponentų tyrimai / Investigations on cells and their biologically active components (FM0202)

Lithuanian Intelligent Specialization

Sveikatos technologijos ir biotechnologijos / Health technologies and biotechnologies (L105)

Author(s)

Author	Affiliation
Zaleskis, Gintaras	Nacionalinis vėžio institutas
Garberytė, Sima	Valstybinis mokslinių tyrimų institutas Inovatyvios medicinos centras
Pavliukevičienė, Božena	Vilniaus universitetas
Krasko, Jan Aleksander	Nacionalinis vėžio institutas
Skapas, Martynas	Valstybinis mokslinių tyrimų institutas Fizinių ir technologijos mokslų centras
Talaikis, Martynas	Vilniaus universitetas
Darinskas, Adas	Nacionalinis vėžio institutas
Žibutytė, Lavija	Nacionalinis vėžio institutas
Pašukonienė, Vita	Nacionalinis vėžio institutas	Chemijos ir bioinžinerijos katedra / Department of Chemistry and Bioengineering (1032)

Title

Comparative evaluation of cellular uptake of free and liposomal doxorubicin following short term exposure

[en]

Gintaras Zaleskis, Sima Garberytė, Božena Pavliukevičienė, Jan Aleksander Krasko, Martynas Skapas, Martynas Talaikis, Adas Darinskas, Lavija Žibutytė, Vita Pašukonienė

Is part of

Anticancer research

Published In

Year	Volume	Issue	Start Page	End Page
2021	41	5	2363	2370

Publisher

Attiki, Greece : International Institute of Anticancer Research

Publisher (trusted)

International Institute of Anticancer Research

Referenced in Database(s)(DBs)

Science Citation Index Expanded (Web of Science)

SCOPUS

Extent

p. 2363-2370

URI

URI	Access Rights
https://hdl.handle.net/20.500.14911/152164
https://doi.org/10.21873/anticanres.15011	Viso teksto dokumentas (atviroji prieiga) / Full Text Document (Open Access)

DOI

10.21873/anticanres.15011

Cited references

Science / Art Area

Gamtos mokslai / Natural Sciences (N)

Field of Science / Art

Biologija / Biology (N010)

Medicina / Medicine (M001)

Biochemija / Biochemistry (N004)

Chemija / Chemistry (N003)

Fizika / Physics (N002)

Keywords (en)

Abstract (en)

Background/Aim: Liposomal Doxorubicin (lipDOX) and free Doxorubicin (DOX) are reported to exhibit similar antitumor efficacy. However, cellular internalization mechanisms of lipDOX are still a subject of controversy. Materials and Methods: Intact and permeabilized cells were exposed for short time to lipDOX and free DOX and drug intracellular content was evaluated by flow cytometry. Then, the antiproliferative capacities of lipDOX and free DOX were compared by the leukocyte nadir test in mice in vivo. Results: The fluorescence increase was 11.2-fold higher in intact cells and 19.7-fold higher in permeabilized cells after exposure to free DOX as compared to lipDOX. Mice injected with DOX showed pronounced antiproliferative activity with a leukocyte count decrease to 2.8±0.65 k/μl (p<0.01) – an effect significantly stronger than that in the lipDOX group. Conclusion: Intact and permeabilized cells internalize free DOX manifold faster than lipDOX. The LipDOX formulation does not induce a remarkable leukocyte nadir effect in vivo.